Q: 1. FFPE sample

FFPE Scrolls (Preferred FFPE sample type): 10 FFPE scrolls, 10 microns thick, with tissue size >5 mm (0.5 cm) or surface area >25 mm 2 FFPE Slides (Only if FFPE scrolls unavailable): 10 unstained FFPE slides, 10 microns thick, with tissue size >5 mm or surface area >25 mm 2 Other formats: Acceptable depending on pathology lab practices. In general, submit material totaling > 100 µm thickness, with individual scroll ≤ 25 µm thick. Block selection: please choose the best block for recut, with the most representative tumor, optimal surface area, minimal necrosis and highest tumor content. A minimum of 10% of tumor fraction is recommended, i.e. neoplastic cells need to comprise at least 10% of the total nucleated cell population. Osteosarcoma cases: please send the most representative sample, preferably tumor-containing non-decalcified soft tissue blocks. Bone specimen should be non-decalcified. If neither soft tissue block nor non-decalcified bone block are available, contact us at CustomerCare@VetOmicsAnimalHealth.com , decalcified bone tissue has been successful with Canine CGP, though non-decalcified sample remains preferred. Labeling & forms: Label all specimen to match name on the VetOmics request form and pathology report. Shipping: FFPE scrolls should be placed in a small Eppendorf tube and shipped at ambient temperature.

Q: 2. FNA Slides

Aspirate and make slides: 5-10 stained or unstained slides. Slides requirement: Slides should have high cellularity and a high percentage of neoplastic cell population , with substantial material , ideally covering >50% of the slide surface rather than a small smear. Cytology review: Perform in-house cytology or submit 1–2 slides for review to confirm adequacy. Labeling & forms: Label slides with the patient’s name and/or VetOmics ID (e.g., VOC25100011) and include the VetOmics confirmation page when shipping. Shipping: Ship slides in a slide box at ambient temperature.

Q: 3. Fresh Frozen Tissue

Collect tissue: Take 2-3 small punch biopsies of the least necrotic, most representative tumor tissue using 2 mm or 4 mm punch tools. Whenever possible, sample tissue adjacent to what is submitted to the pathology lab to ensure the sequenced tumor aligns with the pathologist’s diagnosis. Avoid normal tissue: Minimize collection of grossly normal tissue. NO formalin: DO NOT place the tissue in formalin. If tissue is already in formalin, it must be processed by the pathology lab, and FFPE scrolls should be submitted instead. Tissue preparation: Place the tissue into a small sterile tube (e.g. 5-10 ml tube), no saline needed. Make sure the cap is tightly closed and no leakage. Labeling & forms: Label tube with the patient’s name and/or VetOmics ID (e.g., VOC25100011) and include the VetOmics confirmation page when shipping. Ship and hold WITHOUT dry ice: To submit immediately: ship overnight on ice to our lab. To hold: Freeze the tube in -20 until a decision is made to pursue VetOmics; Ship overnight on ice to our lab. Ship and hold WITH dry ice: To submit immediately: ship tissue on dry ice to our lab. To hold: Freeze the tube in -20 until a decision is made to pursue VetOmics; Ship frozen tissue on dry ice to our lab.

Q: 3. Turn Around Time

Canine CGP Turnaround: 14 days (5-14 days) upon sample arrival

Q: 4. Cost

Canine CGP cost: $900 for hospitals and veterinarians, who may apply hospital markups

Q: 4. EDTA Blood

Collect blood : 2 mL of blood in an EDTA tube (purple top). Labeling & forms: Label the tube with the patient’s name and/or VetOmics ID (e.g., VOC25100011) and include the VetOmics confirmation page when shipping. Shipping: Ship the tube overnight on ice to our lab.

Q: 5. Payment Methods

Payment methods: Credit card (a convenience fee will be added); ACH; Check; Bank Transfer

Q: 5. Urine-based Slides

Collect urine: 40–50 mL urine (catheterized or free-catch). Larger volumes improve yield if cellularity is low. Pellet cells: Centrifuge urine to collect a cell pellet. If the pellet is small or not visible, repeat centrifugation or collect additional urine. Resuspend: Mix the pellet thoroughly in a smaller volume of fluid. Prepare slides: Make 5–10 slides via smearing or cytospin, Slides should have high cellularity and a high percentage of neoplastic cell population, with substantial material, ideally covering >50% of the slide surface rather than a small smear. Cytology review: Perform in-house cytology or submit 1–2 slides for review to confirm adequacy. Labeling & forms: Label slides with the patient name and/or VetOmics ID and include the VetOmics confirmation page when shipping. Shipping: Ship slides in a slide box at ambient temperature.

Q: 6. Effusion-based Slides

Collect sample: Collect effusion fluid. Larger volumes improve yield if cellularity is low. Pellet cells: Centrifuge to pellet cells. If the pellet is small or not visible, repeat centrifugation or collect additional effusion. Resuspend: Mix the pellet thoroughly in a smaller volume of fluid. Prepare slides: Make 5–10 slides via smearing or cytospin, Slides should have high cellularity and a high percentage of neoplastic cell population, with substantial material, ideally covering >50% of the slide surface rather than a small smear. Cytology review: Perform in-house cytology or submit 1–2 slides for review to confirm high cellularity and neoplastic cell percentage. Labeling & forms: Label slides with the patient name and/or VetOmics ID and include the VetOmics confirmation page when shipping. Shipping: Ship slides in a slide box at ambient temperature.

Question 1

1. FFPE sample

vetomicsinternal@outlook.com · Accepted Answer

FFPE Scrolls (Preferred FFPE sample type): 10 FFPE scrolls, 10 microns thick, with tissue size >5 mm (0.5 cm) or surface area >25 mm²
FFPE Slides (Only if FFPE scrolls unavailable): 10 unstained FFPE slides, 10 microns thick, with tissue size >5 mm or surface area >25 mm²
Other formats: Acceptable depending on pathology lab practices. In general, submit material totaling > 100 µm thickness, with individual scroll ≤ 25 µm thick.
Block selection: please choose the best block for recut, with the most representative tumor, optimal surface area, minimal necrosis and highest tumor content. A minimum of 10% of tumor fraction is recommended, i.e. neoplastic cells need to comprise at least 10% of the total nucleated cell population.
Osteosarcoma cases: please send the most representative sample, preferably tumor-containing non-decalcified soft tissue blocks. Bone specimen should be non-decalcified. If neither soft tissue block nor non-decalcified bone block are available, contact us at CustomerCare@VetOmicsAnimalHealth.com, decalcified bone tissue has been successful with Canine CGP, though non-decalcified sample remains preferred.
Labeling & forms: Label all specimen to match name on the VetOmics request form and pathology report.
Shipping: FFPE scrolls should be placed in a small Eppendorf tube and shipped at ambient temperature.

Question 2

1. Request Canine CGP test

vetomicsinternal@outlook.com · Accepted Answer

Registration and Order process:

Create an account at vetomicsanimalhealth.com/login
Stay logged in to place your order
Navigate to the “Order” section.
Click “Order Canine CGP.”
You will be directed to the order form.

Question 3

1. What is Canine CGP?

vetomicsinternal@outlook.com · Accepted Answer

Our flagship test, Canine CGP (Comprehensive Genomic Profiling), is the world’s largest, most advanced tumor genomic diagnostic test available for dogs with cancer. Using whole exome sequencing, Canine CGP evaluates all major mutation types across 20,257 coding genes in the canine genome, providing the most comprehensive view of tumor biology to date to support personalized cancer management and effective treatment.

For comparison, existing commercial tests such as Vidium (~120 genes) and FidoCure (~67 genes) cover only a small fraction of the genome, while other emerging tests assess only a few hundred genes. This difference in genomic coverage translates directly into clinical value with Canine CGP offering:

Higher clinical yield and fewer “empty” results
More treatment options, spanning all targeted therapy, immunotherapy, selected chemotherapy, and radiation
Pharmacogenomic insights (e.g., MDR1 for chemotherapy sensitivity)
Improved diagnosis and prognosis insights
Unparalleled understanding into tumor genome and biology of each tumor

Clinical Performance of Canine CGP

Clinical Performance to date: >300 cases analyzed across 70+ cancer types (as of Oct 2025)

Quality excellence: No case has failed to generate a report. One case required a 2^nd sampling, and 19 cases required a 2^nd workflow attempt, all successfully resolved.
Therapeutic actionability (99.7%): In every case but one, we identified putative pathogenic mutations and clinical actionable biomarkers to support treatment planning. The one negative case was a benign leiomyoma.
Diagnostic value: In over one-third of cases, Canine CGP provided critical diagnostic clarity in challenging scenarios, with diagnostic utility expected to increase as our dataset continues to grow.

Explore these resources to learn how Canine CGP insights translate into clinical practice.

Question 4

2. FNA Slides

vetomicsinternal@outlook.com · Accepted Answer

Aspirate and make slides: 5-10 stained or unstained slides.
Slides requirement: Slides should have high cellularity and a high percentage of neoplastic cell population, with substantial material, ideally covering >50% of the slide surface rather than a small smear.
Cytology review: Perform in-house cytology or submit 1–2 slides for review to confirm adequacy.
Labeling & forms: Label slides with the patient’s name and/or VetOmics ID (e.g., VOC25100011) and include the VetOmics confirmation page when shipping.
Shipping: Ship slides in a slide box at ambient temperature.

Question 5

2. Sample Types

vetomicsinternal@outlook.com · Accepted Answer

Full List of Sample types:

FFPE Scrolls (Preferred FFPE sample type): 10 FFPE scrolls, 10 microns thick, with tissue size >5 mm (0.5 cm) or surface area >25 mm²
FFPE Slides (Only if FFPE scrolls unavailable): 10 unstained FFPE slides, 10 microns thick, with tissue size >5 mm or surface area >25 mm²
FNA Slides: 5-10 stained or unstained slides with high cellularity and high neoplastic cell population
Fresh Frozen Tissue (Ship with ice pack or dry ice): 2-3 small punch biopsies of the least necrotic and most representative tumor tissue, avoid gross normal as much as possible
EDTA Blood (Ship with ice pack): 2 ml blood collected in EDTA tube, used for leukemia or as a normal control in other cancers
Urine-based Slides: 5-10 stained or unstained slides with high cellularity and high neoplastic cell population
Effusion-based Slides: 5-10 stained or unstained slides with high cellularity and high neoplastic cell population
Bone marrow Slides: 5-10 stained or unstained slides with high cellularity and high neoplastic cell population

* These are the samples types we’ve successfully processed. Let us know if you have other sample types.

** You will be responsible for requesting samples from pathology labs to send to our lab, except when the sample is at IDEXX. We work with all major pathology labs and have provided clear instructions on what and how to request. Our order system includes 25+ pathology labs.

***If your sample is with IDEXX, we can request the sample on your behalf, just please follow the prompts on the order form.

**** If your pathology lab isn’t listed, or if you need any assistance with requesting samples, please reach out, we’ll be happy to help.

Question 6

3. Fresh Frozen Tissue

vetomicsinternal@outlook.com · Accepted Answer

Collect tissue: Take 2-3 small punch biopsies of the least necrotic, most representative tumor tissue using 2 mm or 4 mm punch tools. Whenever possible, sample tissue adjacent to what is submitted to the pathology lab to ensure the sequenced tumor aligns with the pathologist’s diagnosis.
Avoid normal tissue: Minimize collection of grossly normal tissue.
NO formalin: DO NOT place the tissue in formalin. If tissue is already in formalin, it must be processed by the pathology lab, and FFPE scrolls should be submitted instead.
Tissue preparation: Place the tissue into a small sterile tube (e.g. 5-10 ml tube), no saline needed. Make sure the cap is tightly closed and no leakage.
Labeling & forms: Label tube with the patient’s name and/or VetOmics ID (e.g., VOC25100011) and include the VetOmics confirmation page when shipping.
Ship and hold WITHOUT dry ice:
1. To submit immediately: ship overnight on ice to our lab.
1. To hold: Freeze the tube in -20 until a decision is made to pursue VetOmics; Ship overnight on ice to our lab.
Ship and hold WITH dry ice:
1. To submit immediately: ship tissue on dry ice to our lab.
1. To hold: Freeze the tube in -20 until a decision is made to pursue VetOmics; Ship frozen tissue on dry ice to our lab.

Question 7

3. Turn Around Time

vetomicsinternal@outlook.com · Accepted Answer

Canine CGP Turnaround: 14 days (5-14 days) upon sample arrival

Question 8

4. Cost

vetomicsinternal@outlook.com · Accepted Answer

Canine CGP cost: $900 for hospitals and veterinarians, who may apply hospital markups

Question 9

4. EDTA Blood

vetomicsinternal@outlook.com · Accepted Answer

Collect blood: 2 mL of blood in an EDTA tube (purple top).
Labeling & forms: Label the tube with the patient’s name and/or VetOmics ID (e.g., VOC25100011) and include the VetOmics confirmation page when shipping.
Shipping: Ship the tube overnight on ice to our lab.

Question 10

5. Payment Methods

vetomicsinternal@outlook.com · Accepted Answer

Payment methods: Credit card (a convenience fee will be added); ACH; Check; Bank Transfer

Question 11

5. Urine-based Slides

vetomicsinternal@outlook.com · Accepted Answer

Collect urine: 40–50 mL urine (catheterized or free-catch). Larger volumes improve yield if cellularity is low.
Pellet cells: Centrifuge urine to collect a cell pellet. If the pellet is small or not visible, repeat centrifugation or collect additional urine.
Resuspend: Mix the pellet thoroughly in a smaller volume of fluid.
Prepare slides: Make 5–10 slides via smearing or cytospin, Slides should have high cellularity and a high percentage of neoplastic cell population, with substantial material, ideally covering >50% of the slide surface rather than a small smear.
Cytology review: Perform in-house cytology or submit 1–2 slides for review to confirm adequacy.
Labeling & forms: Label slides with the patient name and/or VetOmics ID and include the VetOmics confirmation page when shipping.
Shipping: Ship slides in a slide box at ambient temperature.

Question 12

6. Effusion-based Slides

vetomicsinternal@outlook.com · Accepted Answer

Collect sample: Collect effusion fluid. Larger volumes improve yield if cellularity is low.
Pellet cells: Centrifuge to pellet cells. If the pellet is small or not visible, repeat centrifugation or collect additional effusion.
Resuspend: Mix the pellet thoroughly in a smaller volume of fluid.
Prepare slides: Make 5–10 slides via smearing or cytospin, Slides should have high cellularity and a high percentage of neoplastic cell population, with substantial material, ideally covering >50% of the slide surface rather than a small smear.
Cytology review: Perform in-house cytology or submit 1–2 slides for review to confirm high cellularity and neoplastic cell percentage.
Labeling & forms: Label slides with the patient name and/or VetOmics ID and include the VetOmics confirmation page when shipping.
Shipping: Ship slides in a slide box at ambient temperature.

Question 13

6. Where to ship samples to?

vetomicsinternal@outlook.com · Accepted Answer

After submitting the test request, please ship sample to:

James Snyder & Fengxiang Wang
ATTN: VetOmics
ARC 1014, Center for Applied Genomics
Children's Hospital of Philadelphia
3615 Civic Center Blvd
Philadelphia, USA, 19104
Email: CustomerCare@VetOmicsAnimalHealth.com

Question 14

7. Bone Marrow Slides

vetomicsinternal@outlook.com · Accepted Answer

Collect bone marrow and make slides: 5-10 stained or unstained slides.
Slides requirement: Slides should have high cellularity and a high percentage of neoplastic cell population, with substantial material, ideally covering >50% of the slide surface rather than a small smear.
Cytology review: Perform in-house cytology or submit 1–2 slides for review to confirm adequacy.
Labeling & forms: Label slides with the patient’s name and/or VetOmics ID (e.g., VOC25100011) and include the VetOmics confirmation page when shipping.
Shipping: Ship slides in a slide box at ambient temperature.

Question 15

Q1: Bone cancer: No non-decalcified bone sample available for Canine CGP analysis

Accepted Answer

As of October 2025, we have successfully processed multiple decalcified bone tumors and obtained clinically meaningful insights to guide treatment. While the quality may not match that of standard samples and success cannot be guaranteed, these results give us confidence that decalcified specimens can still provide valuable data. Therefore, although non-decalcified bone samples remain preferred, Canine CGP can be attempted when only decalcified samples are available. If the workflow is successful, the standard cost will apply. If we are unable to obtain usable DNA and library to generate data, we’ll know early in the workflow. In that case, we would only charge the actual costs incurred from the service provider. In either case, the pathology lab should choose the best bone block for scroll recut.

Question 16

Q2: Choosing Primary vs. Metastatic Tissue for Canine CGP Analysis

vetomicsinternal@outlook.com · Accepted Answer

We’ve received many questions about whether to analyze primary or metastatic tissue. While each case is unique, here are some general guidelines:

If the lymph node or other mets tissue is sampled at the same time as the primary tumor, we’ll use whichever sample has the best quality. For example, if the primary tumor shows extensive necrosis or low tumor cellularity, we would use the lymph node or other mets tissue. If quality is similar, we typically prioritize the primary tumor, as it most directly reflects the tumor’s biology at diagnosis and is more representative of different tumor clones.
If the metastatic site is collected much later (e.g. after 6-9 months under treatment), then the mets may provide more up-to-date and clinically relevant information on mutation profiles, including resistant clones, so in that scenario we would favor the metastasis.

Question 17

Q3: Leukemia Sampling Consideration

vetomicsinternal@outlook.com · Accepted Answer

Sample type: For leukemia cases in full blast phase, submit 2 mL EDTA whole blood, shipped overnight on ice packs.
Assay sensitivity: Our assay detects neoplastic populations at ≥10% of nucleated cells. Provide an estimate of blast percentage from CBC, differential, smear, or flow cytometry.
Estimating blast fraction:
1. CBC with differential: Initial estimate.
1. Peripheral blood smear: Morphologic blast count is helpful.
1. Flow cytometry: Gold standard for blast percentage and immunophenotype.
1. Bone marrow aspirate: Definitive blast percentage, if available.
If blast fraction <10%: Consider:
1. Collect fresh bone marrow or a later peripheral blood sample during higher disease burden.
1. Perform leukocyte enrichment (e.g., Ficoll/PBMC) or cell sorting before DNA isolation.
1. Accept lower sensitivity and annotate the report accordingly.
Labeling & forms: Label slides with the patient’s name and/or VetOmics ID (e.g., VOC25100011) and include the VetOmics confirmation page when shipping.
Shipping: Ship the tube overnight on ice to our lab.

Question 18

Q4: Feline CGP inquiry

vetomicsinternal@outlook.com · Accepted Answer

Feline CGP covers 16,653 genes using the latest feline reference genome (Fsa126), making it the most comprehensive genomic profiling solution currently available for both research and clinical use. Although the feline genome is less annotated than the canine genome, cats are genetically closer to humans, and our Feline CGP assay performs at a highly robust and reliable level, comparable to our Canine CGP.

Currently offered as an early-release beta product, Feline CGP has consistently identified putative pathogenic mutations and therapeutic targets in feline cancers. However, the use of certain drugs in cats remains an area of active exploration. We are therefore maintaining a soft launch until more dosing data become available.

We look forward to collaborating with you to advance our understanding and improve treatment options for feline patients. We invite you to submit challenging cases to help us explore new therapeutic possibilities and gather real-world data on drug use, all while ensuring the least harm to patients.

Sample requirement:

For now, we need both normal and tumor sample from the same cat. This is different from Canine CGP, where we only need tumor sample. Ultimately, Feline CGP will transition to tumor-only assay, same as Canine CGP

Tumor sample sources, same as for Canine CGP

FFPE Scrolls (Preferred FFPE sample type): 10 FFPE scrolls, 10 microns thick, with tissue size >5 mm (0.5 cm) or surface area >25 mm²
FFPE Slides (Only if FFPE scrolls unavailable): 10 unstained FFPE slides, 10 microns thick, with tissue size >5 mm or surface area >25 mm²
FNA Slides: 5-10 stained or unstained slides with high cellularity and high neoplastic cell population
Fresh Frozen Tissue (Ship with ice pack or dry ice): 2-3 small punch biopsies of the least necrotic and most representative tumor tissue, avoid gross normal as much as possible
EDTA Blood (Ship with ice pack): 2 ml blood collected in EDTA tube, used for leukemia or as a normal control in other cancers
Urine-based Slides: 5-10 stained or unstained slides with high cellularity and high neoplastic cell population
Effusion-based Slides: 5-10 stained or unstained slides with high cellularity and high neoplastic cell population
Bone marrow Slides: 5-10 stained or unstained slides with high cellularity and high neoplastic cell population

Normal sample sources

Normal samples are used as references to distinguish tumor-specific alterations. Please ensure that samples are collected from tissue or sites with no macro- or microscopic tumor involvement, ideally from an anatomically separate location to minimize the risk of tumor contamination.

EDTA blood (1–2 mL): acceptable if the case is not leukemia or lymphoma.
Fresh normal tissue: collected during surgery from a site far away from the tumor; freeze if not shipping immediately.
Skin punch biopsy: from normal skin.
FFPE block: of normal tissue.
Cheek swab: acceptable if not an oral tumor. For swabs, rinse into saline, make slides, and confirm absence of tumor by in-house cytology.

Cost: $900 for clinicians (for two specimens; discounted to support data and insight generation.)

Registration and Request Feline CGP:

Create an account at vetomicsanimalhealth.com/login
Stay logged in to place your order
Navigate to the “Order” section.
Click “Order Canine CGP.”
You will be directed to the order form.
For Feline CGP, order the same way as Canine CGP, except:
1. In Breed, select “OTHER”, enter “Feline: breed”.
1. In Diagnosis, select “OTHER”, enter “Feline: diagnosis"

Question 19

Q5: Handling and Shipping FFPE Scrolls from Pathology Labs

vetomicsinternal@outlook.com · Accepted Answer

Different pathology labs handle scrolls differently, some lab places one scroll per tube, while most group scrolls together. In most cases, we receive 1–2 small tubes containing 5–10 scrolls each.

Using a small Eppendorf tube is preferred, as it helps prevent the scrolls from breaking during shipping.

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